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Description:Tephritidae Bulletin of Entomological Research. Next, internal polymorphisms in each amplified recombinant DNA molecule are genotyped by hybridization with allele-specific oligonucleotides ASOs to map the location of the crossover breakpoint. Note that different studies used different methods to correct recombination assays for amplifications efficiencies, so measured crossover frequencies may be underestimated to different degrees for specific hot spots see the Materials and Methods for further details. The Tephritidae family of insects includes the most important agricultural pests of fruits and vegetables, belonging mainly to four genera Bactrocera, Ceratitis, Anastrepha and Rhagoletis.
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